A Rapid Clinical Method for the Estimation of Urea in Urine
نویسنده
چکیده
An enzyme solution exerting this activity is easily and conveniently prepared from the soy bean (Glycine hispida).4 By extracting the ground tissue with acidified water and filtering, a perfectly clear solution is obtained which under proper precautions can be preserved for a considerable length of time. A solution in which urea is to be determined is treated with a portion of this extract and, after the conversion into ammonium carbonate is complete, the alkalinity of the solution is determined by titration with standard acid using an indicator which is not appreciably sensitive to
منابع مشابه
Estimation of Alpha-amino Nitrogen in Plasma and Urine by the Colorimetric Ninhydrin Reaction.
Methods are described for the estimation of plasma and urine alpha-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both ammonia and urea are separated by a rapid ion-exchange resin procedure. Two methods of estimation are given; one has the advantage of great simplicity, but involves the use of cyanide; in the other, cyanide is eliminated. Normal values are repor...
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The method outlined in the foregoing. paper for the estimation of urea in blood is perfectly reliable for larger quantities of urea (up to 25 mgms. or perhaps more). Its adaptation to the determination of urea in urine using only a small amount of the urine is shown in this paper. The method to be described is slightly more accurate than the rapid clinical method proposed earlier.’ The reason f...
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